Add MESF - Molecules of Equivalent Soluble Fluorochromes

[Lloyd McKenzie]

"MESF units are a relative fluorescence intensity unit which compares a stained sample to the fluorescence intensity of a known solution of equivalent fluorochrome molecules" 7000 hits on google for "Molecules of Equivalent Soluble Fluorochromes"

[Gunther Schadow]

sources? yes, I know it is described here: ​http://www.cyto.purdue.edu/cdroms/cyto4/7_spon/fcsc/mesf.htm But does anyone actually report these? Or are they internal standards that have no meaning when communicated between experiments? Any paper or collaborative effort where laboratories exchange these quantities and they have a reproducible quantitative meaning?

[Brenée Mitchell]

Comment from Dan Vreeman:

This proposal seems reasonable, though not particularly common. I found one scientific paper describing it's use in reporting ZAP-70 levels (​https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.20078) and the calibration kit from Bangs Labs (​https://www.bangslabs.com/sites/default/files/imce/docs/SS%20Quantum%20MESF%20Web.pdf).