Opened 9 years ago

Last modified 7 months ago

#137 assigned enhancement

Add MESF - Molecules of Equivalent Soluble Fluorochromes

Reported by: Lloyd McKenzie Owned by: Daniel Vreeman
Priority: minor Milestone:
Component: help Keywords: flowcytometry
Cc:

Description

"MESF units are a relative fluorescence intensity unit which compares a stained sample to the fluorescence intensity of a known solution of equivalent fluorochrome molecules" 7000 hits on google for "Molecules of Equivalent Soluble Fluorochromes"

Change History (4)

comment:1 Changed 8 years ago by Gunther Schadow

sources? yes, I know it is described here: http://www.cyto.purdue.edu/cdroms/cyto4/7_spon/fcsc/mesf.htm But does anyone actually report these? Or are they internal standards that have no meaning when communicated between experiments? Any paper or collaborative effort where laboratories exchange these quantities and they have a reproducible quantitative meaning?

comment:2 Changed 8 years ago by Gunther Schadow

Keywords: flowcytometry added

comment:3 Changed 9 months ago by Brenée Mitchell

Component: help
Owner: set to Dan Vreeman
Status: newassigned

comment:4 Changed 7 months ago by Brenée Mitchell

Comment from Dan Vreeman:

This proposal seems reasonable, though not particularly common. I found one scientific paper describing it's use in reporting ZAP-70 levels (https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.20078) and the calibration kit from Bangs Labs (https://www.bangslabs.com/sites/default/files/imce/docs/SS%20Quantum%20MESF%20Web.pdf).

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